These transgenic mice have the polyoma virus middle T antigen (PyVT) under control of the mouse mammary tumour virus (MMTV) promoter/enhancer. Females develop mammary tumours which often (strain dependent) metastasize to the lungs. Males develop mammary tumours with longer latency. Pulmonary metastases are observed in 80-94% of tumour-bearing female mice.
These transgenic mice have the polyoma virus middle T antigen (PyVT) under control of the mouse mammary tumour virus (MMTV) promoter/enhancer. Females develop mammary tumours which often (strain dependent) metastasize to the lungs. Males develop mammary tumours with longer latency. Pulmonary metastases are observed in 80-94% of tumour-bearing female mice.
These transgenic mice express Cre recombinase under the control of the human keratin 14 promoter. Used for activation of conditional GEM alleles in K14+ epithelial lineage.
This transgenic strain expresses P1 Cre recombinase under the control of the MMTV LTR promoter. Used for mammary specific activation of conditional GEM alleles; hormonally regulated. Note there are various lines of MMTV-Cre with different phenotypes.
BLG-Cre: Brca1^f/f: Trp53^+/- mice that carry the beta-lactoglobulin Cre (BLG-Cre) transgene are homozygous for floxed exons 22-24 of the breast cancer 1 (Brca1) allele, and are heterozygous for p53 tumour-suppressor gene (Trp53) deficiency. Expression of the BLG-Cre transgene during lactation in females leads to loss of Brca1 function in the mammary gland. This results in formation of mammary tumours exhibiting high grade central necrosis and metaplastic elements in the form of spindle cell and squamous cell differentiation; as seen in human basal-like breast cancers and BRCA1 mutation carriers. Heterozygosity for the mutant p53 allele accelerates the formation of mammary tumours.
These transgenic mice express the ACTIVATED rat c-neu oncogene (Erbb2/Her2) under the control of the mouse mammary tumor virus (MMTV) long terminal repeat (LTR) promoter. Both females and males develop mammary tumours.
These transgenic mice have the polyoma virus middle T antigen (PyVT) under control of the mouse mammary tumour virus (MMTV) promoter/enhancer. Females develop mammary tumours which often (strain dependent) metastasize to the lungs. Males develop mammary tumours with longer latency. Tumour-bearing females have reduced lung metastasis compared to FVB.
This transgenic strain expresses Cre recombinase under the control of the mouse mammary tumor virus (MMTV) long terminal repeat (LTR) promoter. The MMTV LTR promoter directs a widespread pattern of expression. High levels of recombination have been detected in the virgin and lactating mammary gland, salivary gland, seminal vesicle, skin, erythrocytes, B cells and T cells. Little background recombination was observed in the lung, kidney, liver and brain tissues (less than 10%). The donating investigator indicates that this strain may be suitable for use in applications where it is desirable to delete genes in the virgin and lactating mammary gland, skin, erythroid cells, and other secretory tissues and skin.
This transgenic strain expresses P1 Cre recombinase under the control of the MMTV LTR promoter. Used for mammary specific activation of conditional GEM alleles; hormonally regulated. This is a reporter allele for Cre activity that expresses a non-toxic tandem-dimer red fluorescent protein (2 modified DsRed subunits covalently linked) following Cre-mediated deletion of a floxed neo/stop cassette.
To understand the role of stabilized beta-catenin in tumorigenesis in mice, a PGK-neomycin resistance cassette introduced two loxP sites flanking exon 3 and the inserted cassette. When the germline heterozygotes were crossed with mice expressing Cre recombinase under the control of a sheep beta-lactoglobulin promoter (LGB) in the mammary, the serines and threonine encoded by exon 3 and to be phosphorylated by glycogen synthase kinase 3beta (GSK3beta).
Beta-lactoglobin driven Cre recombinase transgene used for mammary specific activation of conditional GEM alleles; hormonally regulated.
The ZEG transgene contains a CMV enhancer/chicken beta actin promoter, a segment consisting of floxed lacZ and neomycin resistance genes/SV40 polyadenylation signal, and an enhanced GFP reporter gene followed by a rabbit beta-globin polyadenylation sequence. Expression of lacZ in transgenic mice is widespread, with notable exceptions being liver and lung tissue. This expression is observed throughout all embryonic and adult stages.
Beta-lactoglobin driven Cre recombinase transgene used for mammary specific activation of conditional GEM alleles; hormonally regulated.
The ZEG transgene contains a CMV enhancer/chicken beta actin promoter, a segment consisting of floxed lacZ and neomycin resistance genes/SV40 polyadenylation signal, and an enhanced GFP reporter gene followed by a rabbit beta-globin polyadenylation sequence. Expression of lacZ in transgenic mice is widespread, with notable exceptions being liver and lung tissue. This expression is observed throughout all embryonic and adult stages.